The Cross Vermont Trail Association (CVTA) is a non-profit organization dedicated to building a multi-use path across the width of Vermont, following the Winooski River and Wells River valleys. The CVTA works to connect local trails into a statewide network for walking, biking, and other four-season activities, partnering with landowners and local groups to create and manage the trail.

Cycling Kansas City Inc. is a 501(c)3 non-profit organization dedicated to promoting safety for all cyclists in the Kansas City Metro area. The organization actively engages with government and civic officials to lobby for better cycling infrastructure and safer streets. It also supports the community through rider education, public service announcement campaigns, and donations to organizations that improve cycling.

The Delaware & Hudson Rail Trail is a 19.8-mile, multi-use trail in two sections, open for walking, cycling, and more. It is a four-season recreation and transportation corridor that travels through scenic countryside in western Rutland and Bennington counties. It is managed by the Vermont Rail Trails Program under the Vermont Agency of Transportation.

Evergreen Mountain Bike Alliance is a 501(c)(3) non-profit organization, and the largest statewide mountain bike association in Washington. It is committed to creating, maintaining, and protecting sustainable mountain biking opportunities for all riders through trail building, advocacy, education, and volunteerism.

The Flagstaff Biking Organization (FBO) is a tax-exempt 501(c)(3) non-profit dedicated to promoting bicycling as a safe and attractive means of transportation and recreation in Northern Arizona. FBO engages in advocacy, trail maintenance, safety education, and community events like the annual "Ride Your Bike Week." They work with local government and forest services to develop and maintain the Flagstaff Loop Trail and other cycling infrastructure.

The Florida Bicycle Association is a 501(c)(3) non-profit organization that works statewide to make the Sunshine State better for people who bicycle. They mobilize, educate, and advocate for policies, projects, and programs that support bicycling and improve safety for all cyclists.

Friends of Arkansas Singletrack (FAST) is a 501(c)(3) non-profit organization dedicated to mountain bike advocacy, education, and trail stewardship in Northwest Arkansas. They organize trail workdays and weekly group rides for all skill levels, working with local stakeholders to elevate the mountain biking community.

Friends Of The Pony Wagon Bike Trail is an all-volunteer, non-profit 501(c)(3) organization dedicated to developing, building, maintaining, and improving a multi-use path in St. Paris, Ohio. Their mission is to create a safe, designated trail for bicycling and walking, with the long-term goal of connecting St. Paris to the vast bicycle path network in Ohio and beyond.

Georgia Bikes, now operating as Go Georgia, is a 501(c)(3) non-profit organization dedicated to advancing safe, equitable, and sustainable transportation and recreation across the state. Originally founded in 2003 to advocate for cyclists, the organization has expanded its mission to include all forms of active transportation, such as walking and rolling, to promote thriving and livable communities in Georgia. They work on local, state, and national levels to improve infrastructure, educate the public on safety and rights, and support local advocacy groups.

The Greater Arizona Bicycling Association (GABA) is a non-profit organization dedicated to promoting and encouraging bicycle riding for recreation, fitness, and transportation. They offer local day rides and multi-day rides in Arizona and New Mexico. GABA also partners with local organizations to teach bike and road safety skills, advocate for cyclists' rights, and develop infrastructure for a bike-friendly culture.

GVORBA is a 501(c)(3) non-profit organization dedicated to serving the greater Greenbrier Valley of West Virginia. Their mission is to create, enhance, and preserve great trail experiences for bikers while promoting a bicycle-friendly community through cycling awareness, safety advocacy, and promotion of bicycling for fun, fitness, and transportation.

The Horner Cycling Foundation is a non-profit organization established in 2023 by Chris and Megan Horner to create an inclusive and accessible junior road cycling team in Central Oregon. The foundation focuses on skill development, team building, and fostering a lifelong love for cycling, particularly addressing the lack of road cycling programs for youth in the area. It provides elite coaching, offers a "bike library" for equipment access, and organizes free races and training sessions.

Bicycle Indiana is a statewide nonprofit bicycle advocacy organization working to make riding a bicycle safe and equitable for all Hoosiers. They achieve this by educating bicyclists, motorists, and policymakers, and advocating for laws, policies, and infrastructure.